Test Kit For Forensic Applications

PCR arrays with accessory components for sample library quantification prior to next-generation sequencing

The QIAseq Library Quant System is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Features

Ready-to-use, predispensed, sequentially diluted DNA standard (array format)

Automatic calculation of library concentration

Simple protocol, high sensitivity, and wide dynamic range

Compatibility with Illumina and Ion Torrent/Proton NGS platforms

Compatibility with most qPCR instruments

Product Details

The QIAseq Library Quant System provides a simple, out-of-the-box solution to quantify NGS libraries, enabling consistent results with every NGS run. The QIAseq Library Quant System uses real-time PCR to specifically quantify DNA molecules with sequencing adaptors at both ends, ensuring optimal cluster density or template-to-bead ratio.

Performance

The sensitivity and dynamic range of the QIAseq Library Quant System were compared against Agilent's High Sensitivity DNA Kit, using two NGS libraries of differing concentrations. The first library was quantifiable by both systems, but the low concentration of the second library was below the detection limit of Agilent's 2100 BioAnalyzer.

Principle

Accurate quantification of library molecules is essential for the efficient use of NGS platforms; however, current spectrophotometric methods only measure total nucleic acid concentrations. The QIAseq Library Quant System, by contrast, uses real-time PCR to quantify only DNA molecules with adaptors at both ends,