CDNA Synthesis Reagent Kit

MicroRNAs are a class of non-coding RNAs with a length of about 22 nt, which play an important role in the regulation of gene expression in plants and animals. This kit uses Poly(A)-tailing method to perform reverse transcription from miRNA first-strand to cDNA. The 2×Hifair™ miRNA RT buffer in the product contains all the raw materials and primers for miRNA Poly(A)-tailing reaction and reverse transcription reaction. Careful optimization ensures that Poly(A) modification process and reverse transcription process can be performed simultaneously and efficiently in miRNA 3' end.
This product is recommended to be used in conjunction with our company's Hieff™ miRNA Universal qPCR SYBR Master Mix (Cat#11171) for optimal experimental results.

Features

High sensitivity detection rate: 10 pg RNA can be detected
High product stability: remains unaffected at 37℃ for 7 days

Applications

First-strand cDNA is synthesized and can be used as a template for RT-PCR and real-time RT-qPCR.

Specifications

Final product type - cDNA (First-Strand)
PCR method - RT-PCR
Reaction format - Isolated fraction
Sample type - miRNA
Optimal reaction temperature - 37℃
Reverse transcriptase - MMLV

Name

Hifair™ miRNA RT enzyme mix - 87.5 μL
2×Hifair™ miRNA RT buffer - 250 μL
RNase-free H2O - 10 mL
Universal Reverse Primer (10 μmol/L) - 4 mL
U6 Forward Primer (10 μmol/L) - 500 μL
U6 Reverse Primer (10 μmol/L) - 500 μL