CDNA Synthesis Reagent Kit

NZY First-Strand cDNA Synthesis Flexible Pack includes all the necessary components to synthesize first-strand cDNA from an RNA template. In addition, it offers the possibility to choose the primer to initiate the reaction.

Details

NZY First-Strand cDNA Synthesis Flexible Pack is an optimized pack that includes the following components: the modified recombinant form of the Moloney Murine Leukemia Virus (MMuLV) Reverse Transcriptase (RNase H minus), which synthesizes the complementary DNA strand using either RNA or single-stranded DNA as template in a wide range of temperatures (37-50 °C); an optimized Reverse Transcriptases buffer; the NZY Ribonuclease Inhibitor for RNA protection against degradation due to ribonuclease contamination; a dNTPs mix; random hexamers and oligo(dT)18 primers; and finally, an E. coli RNase H to specifically degrade the RNA template in cDNA-RNA hybrids after the first-strand cDNA synthesis. This flexible pack offers the possibility to choose the primer to initiate the reaction: you can choose either the primers provided in separate tubes, or a gene-specific primer. The pack is sufficient for up to 200 first-strand cDNA synthesis reactions and it is optimized for RT reactions over a wide range of total RNA concentrations from 10 pg to 5 μg. Downstream applications include real-time PCR, standard PCR and microarrays.

More Information

Shipping Conditions

Dry Ice

Features

Fast, Flexible primer selection, High-yield, Thermostable

Reaction Temperature

50 °C

Protocol time

Approx. 60 min

Components

– NZY Reverse Transcriptase (200 U/μL)

– 10x Reaction buffer for Reverse Transcriptases

– NZY Ribonuclease Inhibitor (40 U/µL)