Enzyme Reagent

The Magigen Magicscript RT-III is the product of reverse transcriptase that cloned and expressed by gene recombination technology. By transforming part of the structural domains of reverse transcriptase genes of different species into chimeras through genetic engineering, the RNase H activity is also completely lost in RT-III, while its excellent thermal stability remains.

Introduction

The Magigen Magicscript RT-III is the product of reverse transcriptase that cloned and expressed by gene recombination technology. By transforming part of the structural domains of reverse transcriptase genes of different species into chimeras through genetic engineering, the RNase H activity is also completely lost in RT-III, while its excellent thermal stability remains.

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 1 nmol of dTTP into acid-insoluble material in 10 minutes at 37°C using poly(rA)•oligo(dT) as template/primer.

Product Features

- A perfect thermal resistance range: From the range of 37℃ to 65℃, Magicscript Reverse Transcriptase (RT) III keeps the same activity that residual activity does not have obvious decline with increasing temperature.
- Great amplification abilities：Magicscript RT-III that lost RNase H activity completely could generate reverse transcription up to 12 kb in length.
- Strong anti-inhibitor characteristic: Resistances to high salt, heparin, blood and other substances that inhibiting synthesis.