Enzyme Reagent Kit

VeriFi™ Library Amplification Mix is ideal for NGS library amplification workflows and challenging PCRs. Combining a powerful and robust proofreading enzyme, greatly reduced GC-dependent bias, and AptaLock™ hot start technology, this mix enables precise PCR, regardless the target you are sequencing.

A superior proofreading Pfu polymerase in a specially formulated 2x PCR ready mix designed for NGS library amplification with reduced GC bias1. This cutting-edge PCR mix offers market leading performance enabling the acquisition of superior quality datasets with a higher number of unique reads2. The mix can be combined with our library quantification kit during NGS library preparation.

Features:

Low GC bias, ideal for high GC/AT targets

More unique reads per NGS dataset for superior data quality

AptaLock™ hot start technology for maximum sensitivity and specificity

100x higher fidelity than Taq DNA polymerase

Room temperature setup

2x ready mix for minimal pipetting

More Information

VeriFi™ Library Amplification Mix was created as a tool for researchers conducting next generation sequencing (NGS) studies. A key part of PCR-based NGS workflows is the library amplification step during which a DNA or cDNA (in the case of RNA-Seq studies) library has been modified to contain appropriate adaptors for the intended sequencing platform and needs to be amplified before proceeding with the sequencing step.

To ensure accuracy of the final sequencing data, proofreading polymerases used in the library amplification step of an NGS pipeline must have certain key characteristics, which are:

High Fidelity, to minimise PCR errors in nucleotide incorporation.