Buffer Solution Reagent Kit

The Total RNA Isolation Kit (Tissue) provides a fast, simple, and cost-effective method to isolated total RNA from tissue sample. Detergents and chaotropic salt are used to lyse cells and inactivate RNase. The specialized high-salt buffering system allows RNA species to bind to the glass fiber matrix of the spin column while contaminants pass through the column. Impurities are efficiently washed away, and the pure RNA is eluted with Buffer RE without phenol extraction or alcohol precipitation. RNA purified with The Total RNA Isolation Kit is suitable for a variety of routine applications including RT-PCR, cDNA Synthesis, Northern Blotting, Differential display, Primer Extension and mRNA Selection. The entire procedure can be completed within 25-40 minutes.

Applications

RT-PCR of RNA.
Northern blotting.
Real-time RT-PCR.

Quality Control
The quality of the Total RNA Isolation Kit (Tissue) is tested on a lot-to-lot basis to ensure consistent product quality.

Size: 100 Reactions

Sample: Up to 30 mg of fresh tissue
Up to 25 mg of paraffin-embedded tissue
Format: Spin column
Operation time: 25-40 minutes
Elution volume: 50 μl
Yield: up to 30 μg

Features

Delivers high-quality total RNA with the fast procedure. Ready-to-use RNA for high performance in any downstream application.
Consistent RNA yield from the starting material with a small amount.

Kit Contents

Contents - Size
Buffer RR - 45 ml
Buffer W1 - 45 ml
Buffer W2 (add Ethanol) - 15 ml (60 ml)
Buffer RE - 10 ml
RT Column - 50 pieces x 2
Collection Tubes - 50 pieces x 2