Solution Reagent Kit

Quick, 5-minute clean-up of large-sized DNA from any enzymatic reaction or impure preparation without messy precipitations.
Unique spin column for low volume elution of ultra-pure, high-yield DNA.
Eluted DNA is ideal for PCR, endonuclease digestion, sequencing, etc.

DESCRIPTION

The Genomic DNA Clean & Concentrator-10 (gDCC) is a gDNA clean up kit that provides quick, 5 minute recovery of ultra-pure, large-sized DNA (e.g., genomic, mitochondrial, plasmid (BAC/PAC), viral, phage, WGA DNA, etc.) from any enzymatic reaction or impure preparation (e.g., Proteinase K digestion). There is no need for organic denaturants, chloroform, or messy precipitations: simply add the specially formulated ChIP DNA Binding Buffer to a sample and then transfer the mixture to the supplied Zymo-Spin Column. Eluted DNA is suitable for sequencing, PCR, endonuclease digestion, and other enzymatic procedures. This gDNA clean up kit is also compatible with smaller DNAs (50 bp to 10 kb) from PCR, digestions, crude plasmid preparations, cDNA synthesis, etc.

TECHNICAL SPECIFICATIONS

Applicable ForEluted DNA is ideal for ligation, sequencing, labeling, PCR, microarray, transfection, transformation, restriction digestion procedures, and any other sensitive downstream application

Elution Volume≥ 10 µl of DNA Elution Buffer

EquipmentMicrocentrifuge

PurityA260/A280 > 1.8, A260/A230 > 1.8

Sample SourceEnzymatic reactions or impure preparations containing genomic DNA.

Size Range50 bp to 200 kb

YieldRecovery of DNA ranges from 70 - 95%