Solution Reagent Kit

The AmpliSeq for Illumina Cancer Hotspot Panel v2 is a targeted resequencing assay for researching somatic mutations across the hotspot regions of 50 genes with known associations to cancer, as identified in the Catalogue of Somatic Mutations in Cancer (COSMIC)1 database. Key features include:

Relevant Gene Content

Target ~2800 COSMIC mutations from 50 oncogenes and tumor suppressor genes

Fast, Streamlined Workflow

Prepare sequencing-ready libraries in a single day from as little as 1 ng of high quality or 10 ng of formalin-fixed, paraffin-embedded (FFPE) samples

Accurate Data

Detect somatic mutations down to 5% variant allele frequency using local or cloud-based analysis

The Cancer HotSpot Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.

This panel enables highly sensitive variant detection within multiple cancer types, including lung, colon, breast, ovarian, melanoma, and prostate. The ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.

Specifications

Assay time - 5 hours (library prep only; doesn't include library quantification, normalization, or pooling time)

Cancer type - Pan-cancer, Solid tumor

Content specifications - Hotspot regions of 50 genes with known associations to cancer

Description - Somatic analysis research into hotspot regions of 50 cancer-related genes.

Hands-on time -