Research Reagent Kit

Faster than ever – under 1-hour RT-PCR cycling protocol
Superior sensitivity – detect as little as 0.1 pg of RNA
Discover the convenience of multiplexing – amplify up to 5 targets within one reaction
Prevent carry-over contamination with preblended UNG enzyme
Enhance sensitivity and tackle GC-rich amplicons with AmpliBoost™ RT-PCR enhancer
Inherently stable due to proprietary Stability TAG technology
Includes inert loading dyes for streamlined gel electrophoresis analysis

SolisFAST® 1-step RT-PCR Kit with UNG provides a simple and streamlined workflow for analyzing RNA targets using reverse transcription PCR (RT-PCR). Both the cDNA synthesis and PCR are conveniently performed in one tube greatly simplifying the analysis workflow, reducing reaction-to-reaction variation, minimizing hands-on steps and reducing reaction set-up to results time.

Exceptionally fast and robust SolisFAST® polymerase combined with our highly processive reverse transcriptases make SolisFAST® 1-step RT-PCR Kit with UNG the fastest kit on the market to perform RT-PCR with unparalleled under 1-hour reaction times. Optimized composition ensures superior sensitivity allowing detection of as little as 0.1 pg of RNA and enabling efficient multiplexing of up to 5 RNA targets simultaneously.

Combination of our highly thermostable FIREScript® and SOLIScript® reverse transcriptases allow efficient reactions at temperatures up to 60 °C to facilitate amplifying difficult RNA targets with challenging secondary structures. The RT mix is preblended with our in silico-designed ribonuclease inhibitor RiboGrip® to minimize the risk of RNA degradation.