Lung Cancer Detection Kit

Human ROS1 gene fusion detection kit (PCR-fluorescence probe method)

This product is used for qualitative detection of formalin-fixed paraffin-embedded FFPE samples of non-small cell lung cancer (NSCLC) in vitro14 ROS1 genes in this RNA are fused (ROS1 gene Exon 32 (COSF 1260/1197/1203/1266/1279)Exon 34（COSF 1261/1198/1201/1280/1268）、Exon 35（COSF 1270/1274/1251）、Exon 36（COSF 1295））

ROS1 gene encodes a tyrosine kinase belonging to the class II RTK insulin receptor family. The ectopic expression of ROS1 and the variant activation of ROS1 kinase can be seen in many tumors, such as glioblastoma multiforme, non-small cell lung cancer and extrahepatic cholangiocarcinoma, showing that ROS1 kinase activation inducesabnormal proliferation and survival of cells. After the fusion rearrangement of ROS1 gene with SLC34A2, CD74, EZR and other genes, the tyrosine kinase region at the C-terminal of ROS1 protein will continue to activate, and continue to act on the downstream signal transduction pathways such as RAS-MAPK/ERK, PI3K/AKT/mTOR, JAK/STAT3, and then lead to cell carcinogenesis [1]. Studies have shown that ALK kinase inhibitors can inhibit the autophosphorylation of Tyr2774 site by competitively binding to ROS1 kinase binding region, thereby blocking the downstream signal pathway induced by ROS1 activation [2,3,4].