Loading Buffer Reagent

A.General Description
SuperCut™ XhoⅠ
Cat. No.: 8052441

Restriction Enzyme Site
5'…C↓T C G A G…3'
3'…G A G C T↑C…5'
Isoschizomers*:PaeR7I，TliI，BssHI，Sfr274I，SlaI，StrI
*Isoschizomers may have different methylation sensitivities.

SuperCut™ quick enzymes are a series of genetically engineered restriction enzymes that can accurately complete DNA digestion in 5~15 minutes, suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA.
SuperCut™ quick enzymes have the following characteristics: digestion can be completed within 5~15 minutes; sharing a digestion buffer, which greatly simplifies the digestion reaction system; good enzyme activity redundancy for easy substrate overload or complex template digestion. In addition, the dephosphorylation and ligation re-agent of BiosciTM is 100% active in SuperCut™ Buffer, sup-porting one-tube reaction and improving the experience of \"digestion-modification-ligation\".

B. Recommended Reaction Conditions
1× SuperCut™ Buffer；
Incubate at 37℃；
Refer to \"Protocol for Fast DNA Digestion\" for reaction setup.

C. Heat Inactivation
1. Incubation at 80℃ for 20 minutes.
2. Add an appropriate amount of 6 × Gel Loading Buffer according to the reaction system to terminate the reaction.

D. Product Composition
Components Amount
SuperCut™ XhoⅠ (10 units/μl) 5000 units
SuperCut™ 10× Buffer 3×1 ml
6× Gel Loading Buffer 1 ml

E. Storage Condition
Storage at -20℃ and validity for 24 months.
Shipped on Blue Ice.