Research Assay Kit

Fast add-and-read protocol performed directly on cells

Luminescent detection with just a plate-reading luminometer

Scalable to 96- or 384-well plates

Quantify Cytokine Release Without Tedious Wash Steps

The Lumit™ IL-4 Immunoassay quantitatively measures released IL-4 in cell culture samples using a simple, no-wash protocol. Just add labeled antibodies to the sample, add detection reagent and read luminescent signal using a standard plate-reading luminometer. The entire protocol is completed in less than 70 minutes! The assay can be used directly on cells in culture or culture medium transferred to a separate assay plate.

Primary antibodies to IL-4 were selected for their specific and sensitive detection and labeled with the LgBiT and SmBiT subunits of NanoBiT® Luciferase. In the presence of IL-4, the subunits are brought together to form an active luciferase enzyme. Addition of optimized substrate generates a bright luminescent signal proportional to IL-4 levels.

Large Dynamic Range of Lumit™ IL-4 Detection

The pg/ml to ng/ml linear range of Lumit™ detection covers expected concentrations for most cell models.

Assay

IL-4 Human Immunoassay

Limit of Detection (LOD)

6.7pg/ml (3 SD above background)

Dynamic Range

18.2pg/ml – 25ng/ml

Minimal Detectable Dose (MDD)
4.5pg/ml (2 SD above background)

Sample Type

Cell culture supernatants

Sensitive Quantitation of Low-Level IL-4 Release

When treated with a Cell Stimulation Cocktail (CSC), T helper type 2 (Th2) cells and human PBMCs (PBMC) each release low levels of IL-4. Following 24 hours of CSC treatment, Lumit™ IL-4 Immunoassay reagents were added directly to cell wells to measure IL-4 release.