Staining Solution Reagent Kit

MicroStacker™ Polymer Detection System Universal is a biotin-free, polymeric horseradish peroxidase (HRP)- secondary antibody conjugate system for the detection of mouse and rabbit primary antibody on formalin-fixed, paraffin-embedded (FFPE) tissues in an immunohistochemistry (IHC) procedure. The innovative MicroStacker™ technology allows well-controlled layered stacking of antibodies and peroxidase enzymes on a micro-polymer scaffold. This technique results in a compact polymeric structure that easily penetrates to all cellular compartments, which provides superior sensitivity compared to other conventional HRP polymers with bulky dextran backbones. Plus, the system utilizes F(ab’) fragments of IgG secondary antibody instead of the whole IgG, which avoids the background caused by non-specific binding of whole IgG to endogenous Fc receptors. After mouse/rat serum cross-adsorption, MicroStacker™ Rabbit-on-Rodent HRP-polymer detection kit can significantly reduce non-specific staining caused by endogenous immunoglobulin. The kit is mainly used for rabbit monoclonal antibody/rabbit polyclonal antibody on mouse/rat tissues staining.

Reagent Composition

Code: RS3000 (3 Components)
MicroStacker™ Anti-Rabbit HRP-Polymer
20*DAB Chromogen
DAB Substrate Buffer

Code: RS3001 (4 Components)
Peroxidase Blocking Reagents
MicroStacker™ Anti-Rabbit HRP-Polymer
20*DAB Chromogen
DAB Substrate Buffer

Code: RS3002 (5 Components)
Peroxidase Blocking Reagents
MicroStacker™ Anti-Rabbit HRP-Polymer
20*DAB Chromogen
DAB Substrate Buffer
Hematoxylin