Extracellular Matrix

The basement membrane is a matrix under the basal surface of epithelial cells of animals. Scilia Extragel Matrix is a reconstituted matrix hydrogel formed by basement membrane components extracted from mouse tumor tissues.

This matrix hydrogel is mainly composed of laminin, collagen IV, and heparan sulfate proteoglycans (Kleinman et al. 1986). Besides, it contains various growth factors, such as epidermal growth factor (EGF), platelet-derived growth factor (PDGF), nerve growth factor (NGF), basic fibroblast growth factor (FGF-2), transforming growth factor-β (TGF-β), and insulin-like growth factor (IGF) (Vu-kicevic et al. 1992).

Product Characteristics
Extragel Matrix is liquid at 4 ℃ but gel-like when heated to 37 ℃. This transformation phemomenon is reversible. It can be liquefied again when it is stored at 4℃ overnight. (Tip: It is recommended to store the Extragel Matrix in an ice box in a refrigerator at 4 ℃ to realize the full liquefaction of the reconstitute matrix hydrogel.)

Storage Condition
Extragel Matrix could be stored in a freezer at -80 ℃ for 2 years.

Product Application
Extragel Matrix can be applied to the growth, differentiation, metabolism and toxicology of organoids, in vivo and in vitro angiogenesis experiments, and tumor formation experiment of immunodeficient mice.

Precautions
Extragel Matrix would start solidifying after the temperature is higher than 10 ℃, so the operation should be performed on ice. The matrix hydrogel can be dissolved in basic culture medium precooled at 4 ℃, and the organoid can be released from the Extragel Matrix.