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This test kit is based on theindirect competitive enzyme immunoassay for the detection of Chloramphenicol in the sample.The coupling antigenis pre-coated on the micro-well stripes. The Chloramphenicol in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Chloramphenicolantibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Chloramphenicol in it. This value is compared to the standard curve and the Chloramphenicol concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 15 ppt
Incubation Temperature: 25℃
Detection limit:
Tissue(method 1)7.5ppt
Tissue(method 2) 15ppt
Egg 15ppt
Recovery rate
Tissue, egg95±25%
Cross-reaction rate:
Chloramphenicol100%
Thiamphenicol< 0.1%
Florfeniol